The SMTP 550 error can happen when a SMTP server rejects email sent to an email address that does not exist.
Example of Error Response:
Final-Recipient: rfc822; <***@gmail.com>
Action: failed
Status: 5.0.0 550_5.1.1_No_such_user_e11si1043640qbc
Diagnostic-Code: smtp; Permanent Failure: Other undefined Status
Last-Attempt-Date: Thu, 17 Aug 2006 12:04:08 -0000